Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae.

Authors: Lin SJ; Defossez PA; Guarente L
Year: 2000
Journal: Science (New York, N.Y.)
Abstract: Calorie restriction extends life-span in a wide variety of organisms. Although it has been suggested that calorie restriction may work by reducing the levels of reactive oxygen species produced during respiration, the mechanism by which this regimen slows aging is uncertain. Here, we mimicked calorie restriction in yeast by physiological or genetic means and showed a substantial extension in life-span. This extension was not observed in strains mutant for SIR2 (which encodes the silencing protein Sir2p) or NPT1 (a gene in a pathway in the synthesis of NAD, the oxidized form of nicotinamide adenine dinucleotide). These findings suggest that the increased longevity induced by calorie restriction requires the activation of Sir2p by NAD.
Reference

Integration:

Created on Nov. 6, 2012, 11:10 a.m.
Not linked
Integrated: False

No notes
Species: Budding yeast

Experiments: 0
Interventions:

MSN2 MSN4 double mutation

PDE2 Deletion

NPT1 deletion

HXK2 deletion

GPR1 deletion

GPA2 deletion

CDC25 mutation

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