lin-4 overexpression | Overexpressing lin-4 extends lifespan [16373574]. | Worm | — | — | — |
lin-4 mutation | Loss-of-function mutation in lin-4 shortens lifespan and accelerated tissue ageing [16373574]. | Worm | — | — | — |
alg-1 RNAi | Adult-specific knockdown of the C. elegans argonaute-like gene 1 *alg-1* results in shortened lifespan with a reduction in the mean and maximum lifespan by 9 - 16% and 14%, respectively [21810936]. | Worm | -9.6 to -16.1 | — | -13.7 |
jnk-1 overexpression | Overexpression of jnk-1 increases lifespan by 40% [15767565; 23097426].
JNK-1 overexpression extends the lifespan in a daf-16-dependent manner. JNK-1 overexpression does not extend the lifespan of animals unable to synthesize miRNAs, i.e. pash-1(mj100) [23097426]. | Worm | +40 | — | — |
lin-14 gain-of-function mutation | A gain-of-function mutation in lin-14 decreases lifespan [16373574]. | Worm | — | — | — |
lin-14 loss-of-function mutation | A loss-of-function mutation in lin-14 extends lifespan by 31% [16373574]. lin-14(n719) mutation extends mean and maximum lifespan of control animals by 20 and 67%, respectively [23097426].
The life-extending effects is dependent on daf-16 and hsf-1 [16373574]. Inactivation of lin-14 does not increase the lifespan of pash-1 mutants [23097426].
| Worm | +20 to +31 | — | +67 |
mir-71 mutation | Loss-of-function of mir-71 decreases lifespan [21129974]. mir-71 mutants have a reduced lifespan with 40% decrease in mean lifespan. Loss of mir-71 function suppresses the long lifespan of glp-1(e2141) mutants [22482727], | Worm | -40 | — | — |
pash-1 mutation | pash-1 mutants have a decreased lifespan and display phenotypic and molecular signs of advantaged age earlier.
pash-1(mj100) temperature sensitive loss-of-function mutation decreases (at the permissive temperature) mean and maximum lifespan by 31 and 71%, respectively. At the restrictive temperature pash-1 mutants are slightly short-lived with a mean and maximum lifespan reduction by 13 and 54%. Lifespan of pash-1 mutants is reduced by a 24h upshift to 25 degree Celsius at the young adult stage with (36 and 78% reduction mean and maximum lifespan, respectively) [23097426].
The rescue of pash-1 expression all tissues restored almost normal lifespan. Rescue specifically in hypodermis, pharynx muscle, gut had no effect on lifespan. Rescue in body wall muscle marginal extended the lifespan, while rescue specifically in the neurons significantly restored mean but not maximum lifespan. Lifespan was also restored by combined rescue in hypodermis and muscle [23097426]. | Worm | -31 | — | -71 |
pyk-1 mutation | pyk-1(ok1754) mutation extends the lifespan and this effect is non-additive with the lifespan extension mediated by DDS treatment [20974969]. | Worm | — | — | — |
DDS treatment | Treatment with DDS either for the entire lifetime or only during the adult period after the L4 stage extends significantly increases mean and maximum lifespan [20974969]
DDS causes the delay of aging, reduces lipofuscin accumulation and decreases the level of a mitochondrial complex as well as lowers oxygen consumption and enhances oxidative stress resistance [20974969].
DDS-conferred lifespan extension is independent of daf-16 and DR (eat-2 mutants) [20974969].
| Worm | — | — | — |
fbxa-121 RNAi | RNA interference of fbxa-121 in adulthood shortens the extended lifespan of daf-2(mu150) mutants. Only a negligible or small reduction in the lifespan of wild-type worms was observed in knockouts [17392428]. | Worm | — | — | — |
Resveratrol supplementation | Resveratrol supplementation prolongs the lifespan [15254550; 17460219], but not in any case [17875315]. | Worm | — | — | — |
SIR-2.4 overexpression | Overexpression of SIR-2.4 does not change the mean or maximum lifespan of wild-type nor the ncreased lifespan of daf-2(e1370) mutants [Tishkoff et al. 2012]. | Worm | — | — | — |
sir-2.4 RNAi | RNA interference against sir-2.4 does not change the mean or maximum lifespan of wild-type not the increased lifespan of daf-2(e1370) mutants [Tishkoff et al. 2012]. | Worm | — | — | — |
let-23 RNAi | Postdevelopmental inactivation of let-23 by RNA interference extends the lifespan by 5.6% [New Longevity Regulators]. | Worm | +5.6 | — | — |
cku-70 RNAi | RNA interference of cku-70 further increases the lifespan of daf-2 mutants. Lifespan of daf-16 mutants is slightly decreased by cku-70 RNAi [16099946]. | Worm | — | — | — |
cyp-42A1 RNAi | RNA interference of cyp-42A1 decreases median lifespan by 39% in wild type animals, 77% in a daf-2 background and 37% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -39 | — |
cyp-33E2 RNAi | RNA interference of cyp-33E2 increases mean lifespan by 18% [17608836]. | Worm | — | — | — |
cwp-4 RNAi | RNA interference of cwp-4 extends lifespan [15998808]. | Worm | — | — | — |
cul-1 RNAi | RNAi of cul-1 decreases lifespan of daf-2 mutant, but not of wild-type or glp-1 mutant. The CUL-1 complex functions in postmitotic, adult somatic tissues of insulin/insulin-like growth factor-1-signaling mutants to enhance longevity. It may act, at least in part, by promoting the transcriptional activity of DAF-16/FOXO [17392428]. | Worm | — | — | — |
cua-1 RNAi | RNA interference of cua-1 decreases median lifespan by 12% in wild type animals and 30% in daf-2 mutants. | Worm | — | -12 | — |
cst-1 overexpression | Overexpression of cst-1 extends lifespan and delays aging in a daf-16-dependent manner [16751106]. | Worm | — | — | — |
cst-1 RNAi | Knockdown of cst-1 shortens lifespan and accelerates tissue aging [16751106]. | Worm | — | — | — |
crn-5 RNAi | RNA interference against crn-5 in adulthood results in a 41% increase in mean lifespan [17411345]. | Worm | +41 | — | — |
col-93 RNAi | RNA interference of col-93 results in extended lifespan [15998808]. | Worm | — | — | — |