| Thor mutation | Null mutation in Thor (alias d4E-BP) causes a significant decrease in longevity (-25% median lifespan in males). foxo (alias dFOXO) and Thor null mutants are compromised in stress resistant. Stress resistance of foxo null mutants is rescued by Thor overexpression [16055649]. Thor null mutants cancel out DR-induced lifespan extension, because mutants exhibit a diminished change in lifespan when nutrient conditions were varied. Thor null mutants have a wild-type similar reduction in egg production upon DR [19804760]. | Fly | — | -25 | — |
| alg-2 RNAi | RNA interference of alg-2 decreases median lifespan by 24% in wild type animals and 50% in a daf-2 background [18006689]. | Worm | — | -24 | — |
| lin-40 RNAi | RNA interference decreases median lifespan by 24% in wild-type animals, 38% in a daf-2 background and 24% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -24 | — |
| sbds-1 RNAi | RNA interference of sbds-1 decreases median lifespan by 24% in daf-2 mutants [18006689]. | Worm | — | -24 | — |
| calu-1 RNAi | RNA interference of calu-1 decreases median lifespan by 23% in a daf-2 background and 15% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -23 | — |
| Nlaz mutation | Absence of Nlaz, which is homologous to ApoD, results in a reduced lifespan in both sexes. Median lifespan is 30.8% and 22.5% lower in females and males, respectively. Maximum lifespan is reduced by 12% and 30% in females and males [21376794]. | Fly | — | -22.5 to -30.8 | -12 to -30 |
| AVT1 deletion | Deletion of AVT1 accelerates the development of age-induced mitochondrial dysfunction without effecting the kinetics of vacuolar acidity decline and prevents the suppression of mitochondrial dysfunction by VMA1 and VPH2 overexpression without affecting vacuolar acidity. AVT1 deletion decreases mean, median and maximum lifespan by 21, 22, and 12%, respectively [23172144]. | Yeast | -20.6 | -22.4 | -11.8 |
| cdk-7 RNAi | RNA interference of cdk-7 decreases median lifespan by 18% in a daf-2 background and 19% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -18 | — |
| HES1 overexpression | Elevation of HES1 levels by an ERG6 promoter reduces mean, median and maximum replicative lifespan by 25, 18 and 29% [Geber et al., unpublished] | Yeast | -25 | -18 | -29 |
| blmp-1 RNAi | RNA interference of blmp-1 decreases median lifespan by 17% in a daf-2 background and 20% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -17 | — |
| mboa-1 RNAi | RNA interference of mboa-1 decreases median lifespan by 16% in wild type animals, 28% in a daf-2 background and 12% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -16 | — |
| Scgdelta deletion | Deletion of Scgdelta has detrimental effects on the flight muscles of adult animals and heart function. Median lifespan is reduced 15-30% [17855453]. | Fly | — | -15 to -30 | — |
| Lnk mutation | Loss of Lnk function results in increased median (14% in females and 17.5 in males) and maximum lifespan, reduced female fecundity and improves survival under conditions of oxidative stress and starvation. Heterozygousity does not result in any significant differences in lifespan in either males or females. Moreover, lifespan extension in one of the female homozygous mutant is fully rescued by the introduction of a Lnk genomic rescue construct [20333234]. | Fly | — | -14 to -17.5 | — |
| C14A4.9 RNAi | RNA interference of C14A4.9 decreases median lifespan by 14% in wild type animals and 41% in daf-2 mutants [18006689]. | Worm | — | -14 | — |
| skn-1 mutation | skn-1 mutation does not alter lifespan under AL, but cancels out the lifespan extension effect of lDR or food variation at all. Response to lDR in skn-1 mutant is restored by ectopic expression of skn-1 in ASI neurons and gut. Ectopic expression of skn-1b in ASI neurons rescued lDR longevity defects of skn-1. lDR worms exhibit elevated respiration, which is absent in skn-1 mutants. skn-1 is necessary for increased respiration and the increase in respiration is necessary for lDR longevity effect, because two different inhibitors of mitochondrial electron transport chain complex III, myxothiazol and antimycin, suppress lDR longevity without shortening lifespan under AL. IF significantly extends lifespan of skn-1 mutants [19079239]. sDR extends lifespan of a skn-1 loss-of-function mutant (which displays a premature stop codon in all three isoforms) and wild-type to a similar extent [19239417]. skn-1(zu67) mutation decreases mean, median, and maximum lifespan by 11-23, 13-28 and 12-23%, respectively, and totally cancels out lifespan extension by ragc-1 RNAi [22560223]. | Worm | -11 to -23 | -13 to -28 | -12 to -23 |
| C26B0.3 RNAi | RNA interference of C26B0.3 decreases median lifespan by 12% in wild type animals, 68% in a daf-2 background and 17% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -12 | — |
| C29F9.2 RNAi | RNA interference of C29F9.2 decreases median lifespan by 12% in wild type animals and 36% in daf-2 mutants [18006689]. | Worm | — | -12 | — |
| cua-1 RNAi | RNA interference of cua-1 decreases median lifespan by 12% in wild type animals and 30% in daf-2 mutants. | Worm | — | -12 | — |
| Sir2 RNAi | Decreased expression of Sir2 and Sir2-like genes in all cells causes lethality during development. Suppression of the Sir2 in neurons decreases the median lifespan by 10-30%, while ubiquitous silencing of the Sir2-like genes shortens lifespan. The effects are server at 28°C that at 25°C [17159295]. | Fly | — | -10 to -30 | — |
| C11H1.3 RNAi | RNA interference of C11H1.3 decreases median lifespan by 10% in wild type animals, 14% in a daf-2 background and 41% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -10 | — |
| mdh-1 RNAi | RNAi against mdh-1 decreases median lifespan by 10% in wild type animals and by 16% in daf-2 mutants. mdh-1 RNAi started after the animal reached the late L4 stage increases mean and maximum lifespan by 4-27% and 9% [22103665]. | Worm | +4 to +27 | -10 | +9 |
| ain-1 RNAi | RNA interference of ain-1 decreases median lifespan by 10% in wild type animals, 20% in a daf-2 background and 44% in daf-2/daf-16 double mutants [18006689]. | Worm | — | -10 | — |
| C60-olive oil treatment | Oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) for just about 7 months to rats not only does not entail chronic toxicity but it almost doubles the lifespan. The effects on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours [22498298].
C60-olive oil can increase the mean, median and maximum lifespan by 114, 91 and 74%. C60-olive oil extends the lifespan of animals with a probability of 0.999 and 0.995 with respect to water and olive oil treatments, respectively [22498298].
The GSSG/GSH ratio of animals treated by C60-oil is significantly less (about twice as less) as compared to controls [22498298].
| Rat | +113.8 | +90.9 | +73.7 |
| Sirt6 overexpression | Overexpression of Sirt6 in male mice lengthens the median lifespan by 9.9-14.5% and maximum lifespan by 13.1-15.8% [22367546]. | Mouse | — | +9.9 to +14.5 | +13.1 to +15.8 |
| fabp overexpression | Overexpression of fabp (CG6783) throughout the whole body increases mean, median and maximum lifespan by 77, 81 and 13%, increases stress resistant (to paraquat but not starvation), consistently reduces mortality rate across adult ages and reduces the lifespan extension of DR by 12% [22997544].
fabp overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of fabp [22997544].
Females of the genotype Act-GS-Gal4 > UAS-CG6783 exhibit an increase in median lifespan compared to uninduced control in response to feeding with RU486-containing food from day 3 of adulthood (P < 0.0001). Mean lifespan is extended by 10, while maximum lifespan is decreased by 11% [22997544]. | Fly | +77 | +81 | +13 |
GenAge
GenDR
