| Sirt2 RNAi | Decreased expression of Sirt2 by RNA interference causes lethality during development. Silencing in neurons shortened mean lifespan by 20% [17159295]. | Fly | -20 | — | — |
| Dcr-2 mutation | Median lifespan of homozyogous and transheterozyogous Dcr-2 mutants is reduced by 18-36% in males and by 27-36% in females. Dcr-2 loss changes the expression of mostly metabolic genes implicated in stress resistance and aging. Dcr-2 mutants are hypersensitive to oxidative, endoplasmic reticulum, starvation and cold stress as well as abnormal lipid and carbohydrate metabolism [21889502].
| Fly | -18 to -36 | — | — |
| Sh mutation | Genetic mutation in Sh decrease lifespan by accelerating the aging proces. At 25 degree mean and maximum lifespan is reduced by 16 and 22%, while by 18 degree Celsius the reduction is 32 and 21% [8582611]. | Fly | -16 to -32 | — | -21 to -22 |
| Akt1 RNAi | RNA interference of Akt1 in intestinal stem cells, results in impaired regeneration of the intestinal epithelium and a short lifespan. In males and females on mean lifespan is 11.4% and 7.4% lower [20976250]. | Fly | -11.4 to -7.4 | — | — |
| g mutation | Loss-of-function mutation reduces mean lifespan by 11 - 42% and maximum lifespan by 7 - 30% [17435236]. | Fly | -11 to -42 | — | -7 to -30 |
| Atg8a mutation | Mutations in Atg8a results in reduced lifespan and increased sensitivity to oxidative stress [18059160].
Atg8a mutation reduces the maximum lifespan by 25% under starvation conditions [17617737].
Loss-of-function mutation in atg8a reduces mean lifespan by 11 - 25% and maximum lifespan by 3 - 22% [17435236]. | Fly | -11 to -25 | — | -3 to -25 |
| bsk RNAi | RNA interference of bsk in intestinal stem cells, results in short lived mutants with impaired intestinal homeostasis and tissue regeneration. The mean lifespan of males is 16.4% lower and those of female is reduced by 10.2% [20976250]. | Fly | -10.2 to -16.4 | — | — |
| InRE19/InRp5545 transheterozygous mutation | Mutations in InR (InRE19/InRp5545 transheterozygous) result in dwarf females with extended lifespan of up to 85% and dwarf males with reduced late age-specific mortality (although no significant change in lifespan) [11292875]. | Fly | +85 | — | — |
| fabp overexpression | Overexpression of fabp (CG6783) throughout the whole body increases mean, median and maximum lifespan by 77, 81 and 13%, increases stress resistant (to paraquat but not starvation), consistently reduces mortality rate across adult ages and reduces the lifespan extension of DR by 12% [22997544].
fabp overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of fabp [22997544].
Females of the genotype Act-GS-Gal4 > UAS-CG6783 exhibit an increase in median lifespan compared to uninduced control in response to feeding with RU486-containing food from day 3 of adulthood (P < 0.0001). Mean lifespan is extended by 10, while maximum lifespan is decreased by 11% [22997544]. | Fly | +77 | +81 | +13 |
| Hsp70 locus overexpression | Overexpression of the Hsp70 locus (containing Hsp70Bb and Hsp70Bc) in transgenic flies extends lifespan as much as 7.9% [9363888]. | Fly | +7.9 | — | — |
| cher overexpression | Overexpression of cher from a doxycycline-inducible promoter results in a 7 - 9% increase in mean lifespan (in PdL x rtTA; Oregon-R x rtTA) [12620118]. | Fly | +7 to +9 | — | — |
| Fwd overexpression | Overexpression of Fwd (from a doxycline-inducible promoter) increases mean lifespan by 7-9% [12620118]. Fwd regulates actin organization and ring canal formation during germline cytokinesis [10934029] by catalysing the first step in the synthesis of the key regulatory membrane phsopholipid PIP2, which is generated from PIP by a PI 4,5-kinase. | Fly | +7 to +9 | — | — |
| CG18809 RNAi | RNAi of CG18809 results in a 7-19% increase in mean lifespan of females, while neural RNAi results in an increased mean lifespan of up to 12% in females. For males the results are variable [19747824]. | Fly | +7 to +19 | — | — |
| Curcumin treatment | In fruit fly, 0.5 an 1.0 mg/g curcumin in the diet increases mean lifespan by 6.2 and 25% in females and by 15.5 and 12.6 in males, respectively. Lifespan extension by curcumin was associated with the increased superoxide dismutase (SOD) activity, upregulation of Ms-SOD and CuZn-SOD genes, and the downregulation of *dInR*, *ATTD*, *Def*, *CecB* and, *DptB* genes as well as reduction of lipofuscin, malondialdehyde and lipid peroxidation [22653297; 23325575]. Curcumin prolongs life and enhances activity of fruit fly Alzheimer diseased flies [22348084]. | Fly | +6.2 to +25 | — | — |
| Cct1 overexpression | Overexpression of Cct1 from a doxycycline-inducible promoter results in a 6 - 8% increase in mean lifespan (in the PdL x rtTA; Oregon-R x rtTA strain) [12620118]. | Fly | +6 to +8 | — | — |
| Rdh overexpression | Overexpression of Rdh from a doxycycline-inducible promoter results in a 6-17% increase in mean lifespan [12620118].
Rdh is an open reading frame in the first intron of the encore gene [12620118]. | Fly | +6 to +17 | — | — |
| Minocycline treatment | Treatment with minocycline (0.87mM) prolongs mean, median and maximum lifespan of wild-type (Oregon strain) of both genders. In females mincocycline extend mean and maximum lifespan by 57 and 78%, respectively. In males minocycline results in a mean and maximum lifespan extension by 114 and 28%, respectively [23185716]. | Fly | +57.1 to +114.3 | — | +28.1 to +78.3 |
| Atg8a overexpression | Enhanced expression of Atg8a in older fly brains extends average adult lifespan by 56% and promotes resistance to oxidative stress [18059160]. | Fly | +56 | — | — |
| Homozygous chico mutation | Mutation in chico extends mean, median, and maximum lifespan by 56%, 48%, and 42% in homozygotes. chico mutation produces dwarf, long-lived females at normal nutrition [11292874]. Wild-type and chico mutant females have similar peak lifespan under DR, but the food concentration at which these are achieved is shifted to higher amounts. chico mutation induces a state equivalent to submaximal, DR-induced slowing of aging [11951037]. Male chico homozygous have a shortened lifespan [11292874]. Female chico homozygous recessive mutants are sterile [11292874]. | Fly | +56 | +48 | +42 |
| Gclc overexpression | Overexpression of Gclc extended mean and maximum lifespan by up to 50% [16148000]. | Fly | +50 | — | +50 |
| ovo mutation | The dominant ovoD1 allele extends female lifespan by approximately 50%. It does not synergize or prevent life-extension caused by chico [10617470; 11292874].
ovoD1 mutants are sterile [Mevel-Ninio et al. 1991]. | Fly | +50 | — | — |
| sug overexpression | Overexpression of sug (from a doxycycline-inducible promoter) results in a 5-9% increase in mean lifespan [12620118]. | Fly | +5 to +9 | — | — |
| hebe overexpression | Adult-specific overexpression of hebe increases the lifespan by 5-30% and modulates late-age female fecundity. Female and male mean lifespan is up to 11% and 24% higher [19011900]. | Fly | +5 to +30 | — | — |
| magu overexpression | Adult-specific overexpression of magu increases lifespan by 5-30% and modulates late-age fecundity [19011900]. | Fly | +5 to +30 | — | — |
| VhaSFD overexpression | Overexpression of VhaSFD (from a doxycycline-inducible promoter) results in a 5-10% increase in mean lifespan [12620118]. | Fly | +5 to +10 | — | — |
GenAge
GenDR
