| sod-1 overexpression | sod-1 overexpression increases mean, median, and maximum lifespan by 32, 25, and 35% [22737090]. | Worm | +32 | +25 | +35 |
| Overexpression of constitutive nuclear SKN-1 | skn-1 transgenes that overexpress a constitutive nuclear form of SKN-1 in the intestine extend the mean lifespan by 5-21%, independently of DAF-16 [18358814]. | Worm | +5 to +21 | — | — |
| sir-2.1 overexpression | sir-2.1 overexpression extends lifespan by about 50% and this lifespan extension depends on DAF-16 activity as it is suppressed by mutation in daf-16 and it does not synergize with daf-2 [11242085].
Overrexpression of sir-2.1 synergizes with TGF-beta mutation (daf-4 and daf-1) for dauer formation [11242085]. | Worm | +50 | — | — |
| Cat overexpression | Simultaneous overexpression of catalase and Sod1 results in a one-third (i.e. 30%) lifespan extension, a slower rate of mortality acceleration, and a delayed loss in physical performance, but neither has any effect on lifespan alone [8108730]. Targeted overexpression of CAT in the mitochondrial matrix has no effect on lifespan at 25 degree Celsius, slightly shortens lifespan at 29 degree Celsius as is associated with increased resistance to endogenous hydrogen peroxide, paraquat, and cold stress [12521602]. | Fly | — | — | — |
| Overexpression of Cat and Sod1 | Simultaneous overexpression of catalase and Sod1 results in a one-third (i.e. 30%) lifespan extension, a slower rate of mortality acceleration, and a delayed loss in physical performance, but neither has any effect on lifespan alone [8108730]. | — | +30 | — | — |
| Sod overexpression | Simultaneous overexpression of catalase and Sod (alias Sod1) results in a one-third lifespan extension, a slower rate of mortality acceleration, and a delayed loss in physical performance, but neither has any effect on lifespan alone [8108730]. General overexpression of Sod (also known as Cu/ZnSOD) alone is sufficient to extend lifespan by up to 48%. Simultaneous overexpression of catalase with Cu/ZnSOD has no added benefit, presumably due to a pre-existing excess of catalase [9858546]. | Fly | +38 | — | — |
| Prx5 overexpression | Prx5 overexpression causes an increase in mean and median lifespan under normal conditions. It also leads to a small increase in maximum lifespan [21826223]. | Fly | — | — | — |
| FPS1S overexpression | Plants overexpressing FPS1S exhibit a cell death/senescence-like phenotype and grw vigorously than wild-type [12000449]. In plants with increased FPS activity, the expression of senescence activated gene SAG12 is prematurely induced. | — | — | — | — |
| Pka-C1 overexpression | PKA-overexpressing flies (hsPKA*/+) have an about 30% extends maximum lifespan [17369827]. | Fly | — | — | +30 |
| pha-4 overexpression | pha-4 overexpression increases longevity of wild-type only slightly, but significant that of daf-16 mutants [17476212]. | Worm | — | — | — |
| CLN3 overexpression | Overexpression shortens chronological lifespan together with age-dependent increases in genome instability and apoptosis. While around 80% of wild-type cells are alive almost non CLN3 overexpressers are alive (under condition that avoids adaptive regrowth) [17710147]. | Yeast | — | — | — |
| ucp-4 overexpression | Overexpression reduces mean lifespan by 5%, has no significant effect on median lifespan, and slighlty increases maximum lifespan by 12% [22737090]. | Worm | -5 | — | +12 |
| p53 overexpression | Overexpression of wild-type Dmp53 during adult life has no significant effect on lifespan [16303568]. | Fly | — | — | — |
| daf-16 overexpression | Overexpression of wild-type DAF-16 modestly increases lifespan by 20% [11747825]. | Worm | +20 | — | — |
| VPH2 overexpression | Overexpression of VPH2 increases the levels of assembled V-ATPase at the vacuolar membrane, increases vacuolar acidity and suppresses age-induced mitochondrial dysfunction of aged cells (17 or 18 cell divisions) which requires the V-ATPase activity. VPH2 overexpression significantly increases mean, median and maximum replicative lifespan by 23, 25 and 34%, respectively [23172144].
| Yeast | +23.1 | +25.0 | +34.0 |
| VMA1 overexpression | Overexpression of VMA1 increases vacuolar acidity and suppresses age-induced mitochondrial dysfunction of aged cells (17 or 18 cell divisions) which requires the V-ATPase activity. VMA1 overexpression significantly increases mean, median and maximum lifespan by 39 - 45%, 39 - 48% and 50 - 60%, respectively. DR (0.5% glucose restriction) does not further increase the lifespan of VMA1 overexpression strain [23172144]. | Yeast | +39.3 to +44.8 | +39.3 to +48.3 | +50.0 to +60.0 |
| VhaSFD overexpression | Overexpression of VhaSFD (from a doxycycline-inducible promoter) results in a 5-10% increase in mean lifespan [12620118]. | Fly | +5 to +10 | — | — |
| PEP4 overexpression | Overexpression of vacuolar aspartyl protease (PEP4) extends chronological lifespan by increasing cytosolic polyamine and S-adenosylmethionine (SAM) levels. Deletion of PEP4 results in both apoptotic and necrotic cell death during chronological aging [21593793]. | Yeast | — | — | — |
| ucp2 overexpression | Overexpression of ucp2 increases mean, median, and maximum lifespan by 42, 40, and 26%, which is non-additive with sDR [22737090]. | Worm | +42 | +40 | +26 |
| uba-1 overexpression | Overexpression of uba-1 does not result in significant increase in median lifespan [22737090]. | Worm | — | — | — |
| TXN overexpression | Overexpression of TXN1 in transgenic C57BL/6 mice resulted in extended median (35%) and maximum (22%) lifespan. Telomerase activity in spleen tissues of TXN1 overexpressing mice is higher than tha in wild-type [12230882]. | Mouse | — | +35 | +22 |
| TrxT overexpression | Overexpression of TrxT in neurons increases the level of locomotor activity in aged flies and extends the mean lifespan by 15% [17301052]. | Fly | +15 | — | — |
| Trxr-1 overexpression | Overexpression of Trxr-1 (alias GSR; glutathione reductase) in transgenic flies results in increased lifespan and oxidative stress resistance, but only under hyperoxia [10506576]. | Fly | — | — | — |
| to overexpression | Overexpression of to adult neurons, pericerbral or abdonimal fat body increases male and female lifespan. to overexpression in the adult nervous system, head fat body and abdominal fat body results in 25, 20 and 12-18% increase of mean lifespan. on average the mean lifesapn is extended for males and females by 18 and 26%, while maximum lifespan of male and female is increased by 13 and 25% [20519778]. | Fly | +12 to +26 | — | +13 to +25 |
| Hsp70 locus overexpression | Overexpression of the Hsp70 locus (containing Hsp70Bb and Hsp70Bc) in transgenic flies extends lifespan as much as 7.9% [9363888]. | Fly | +7.9 | — | — |
GenAge
GenDR
