| MEP3 | — | Deletion of MEP3 extends chronological lifespan [16418483]. | Budding yeast |
| MPT5 | — | Overexpression of MPT5 from the ADH promoter extends replicative lifespan by about 20% in W303R [11805047] and by 25% in PSY142 [9150138], whereas the deletion of MPT5 shortens lifespan by about 50% [9150138; 7859289]. MPT5 deletion decreases average chronological lifespan by 50%, which is rescued to the wild-type level by PKC1 overexpression [17172436].
MPT5 mutants are temperature sensitive [7845352], hypersensitive to mating pheromone [9154842], and null mutants exhibit increased silencing at telomeres and decreased rDNA silencing [9584615]. Deletion of MPT5 is synthetical lethal with mutation of either SWI4, SWI6, or CCR4 in an ssd1-d background [11805047]. MPT5 overexpression suppresses the temperature phenotype of POP2 mutant [9504907]. MPT5 is required for relocalization of the SIR complex to the nucleolus in sir4-42 strain [7859289]. | Budding yeast |
| MXR1 | peptide Methionine sulfoXide Reductase 1 | Deletion of MXR1 (alias MsrA) decreases by 25% and overexpression slightly increases the replicative lifespan [15141092]. Deletion of MXR1 decreases replicative lifespan [19049972]. MXR1 deletion decreases replicative lifespan on either glucose or lactate as carbon source [20799725]. Although deletion or overexpression of MXR2 (alias MsrB) has no effect under normal growth conditions, the simultaneous deletion of MXR1 and MXR2 reduces the lifespan by 63% [15141092]. | Budding yeast |
| NDI1 | NADH Dehydrogenase Internal 1 | Deletion of NDI1 results in chronological lifespan extension [16436509]. | Budding yeast |
| PHB1 | ProHiBitin 1 | Deletion of PHB1 results in a slight reduction in mean and maximum replicative lifespan and a defect in mitochondrial membrane potential. When both PHB1 and PHB2 genes are deleted, the mean replicative lifespan is reduced by one third (30%) that of the wild-type strain [9259555]. Deletion of PHB1 decreases replicative lifespan by 20% [12882345]. Phenotypic changes characteristic of aging cells (e.g. lengthening of cell cycle and specific morphological changes) suggests that PHB1;PHB2 double mutants undergo premature aging, not simply reduction of viability [9259555].
There is no reduction in stress resistance or bulk growth rate in PHB1 mutants. PHB1;PHB2 double mutant have a strong defect in mitochondrial potential, while PHB1 mutant have only a slight defect [9259555]. PHB1 deletion is synthetical lethal with mutation of outer mitochondrial membrane proteins, Mdm12, Mdm10, or Mmm1 [9632789]. | Budding yeast |
| RAD27 | — | Deletion of RAD27 results in signs of premature aging and approximately 60% reduction in mean replicative lifespan [12024027]. | Budding yeast |
| RAD50 | RADiation sensitive 50 | RAD50 mutations result in a 70% reduced replicative lifespan [10207108]. | Budding yeast |
| RAD51 | RADiation sensitive 51 | Rad51 mutations result in a 40% reduced mean replicative lifespan in strain PSY316 [10207108]. RAD51 is required for gene conversion, but not for repair of an HO-induced double-stranded break [8692957]. RAD51 deletion decreases formation of extrachromosomal rDNA circles [10207108]. | Budding yeast |
| RAD52 | RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) | Deletion in RAD52 causes a 75% reduction in mean replicative lifespan in PSY316 strain [10207108]. Similiar reduction of lifespan occurs in strains W3031-A and W303R [M. Baeberlein, M. McVey, and L. Guarente, unpublished].
RAD51 is required for formation of extrachromosomal rDNA circles [10207108], but not for replication fork pausing nor DNA breakage with the rDNA [10693764]. | Budding yeast |
| RAD57 | — | Mutations result in a 40% reduced lifespan. | Budding yeast |
| RAD9 | RADiation sensitive 9 | Deletion results in mutants with a 2-fold decrease in mean and maximum chronological lifespan under conditions of nutrient depletion [17710147]. Mutation of RAD9 shortens lifespan by 30% in DBY747 [7806576] and in strain W303 [11290710].
RAD9 mutation has no effect on telomeric silencing or length [10924458]. | Budding yeast |
| RAS1 | — | Deletion in RAS1 increases mean (23%) and maximum (29%) replicative lifespan (in SP1) [8034612]. RAS1 deletion increases replicative lifespan by 15% in the alpha strain [19030232]. However, deletion of RAS1 slightly shortens chronological lifespan (in SP1) [12586694]. No lifespan extension results from overexpression of RAS1 (in SP1) [8034612]. | Budding yeast |
| RAS2 | Ras-like protein 2 | Overexpression of RAS2 causes a 43% increase in mean and 18% increase in maximum lifespan as well as postpones the age-related increase in generation time. RAS2 deletion causes a 23% decrease in mean and a 30% decrease in maximum lifespan [8034612]. Deletion of RAS2 leads to a longer chronological lifespan [21076178]. Deletion of the RAS2 gene, which functions upstream of CYR1, doubles the mean chronological lifespan by a mechanism that requires Msn2/4 and Sod2 [12586694]. DR further extends chronological lifespan of ras2Delta [18225956]. | Budding yeast |
| REI1 | Cytoplasmic pre-60S factor | REI1 deletion increases mean replicative lifespan by about 40% [16293764] in the alpha and a strains [19030232]. | Budding yeast |
| RIM15 | Regulator of IME2 15 | RIM15 deletion results in 50% reduction of maximal chronological lifespan [11292860] and consistently decreases chronological lifespan under AL [21076178]. Rim15 is required for chronological lifespan extension caused by deficiency in RAS2, TOR1, or SCH9, as well as by 0.5% glucose restriction, but not by water starvation [18225956]. | Budding yeast |
| ROM2 | RhO1 Multicopy suppressor 2 | Deletion of ROM2 increases mean replicative lifespan of the alpha strain by about 50% [16293764]. ROM deletion mutant replicative lifespan increases by 49% in the alpha strain and 16% in a strain. Deletion of ROM2 increases replicative lifespan by 38% in the alpha strain and by 29.3% in the a strain (34.2% in both) [19030232]. | Budding yeast |
| RPD3 | Reduced Potassium Dependency 3 | Deletion of the histone deacetylase gene RPD3 extends lifespan by 41%, independently of an intact Sir silencing complex (in the short lived YSK661 strain) [10512855]. Deletion of RPD3 extends replicative lifespan and there was no additive effect by neither 0.1% glucose nor amino acid restriction [12213553].
RPD3 deletion increases rDNA silencing in a partially SIR2-dependent manner [10082585].
Its effects on chromatin functional state were evidenced by enhanced silencing at the three known heterochromatic regions in the genome, the silent mating type (HM), subtelomeric, and rDNA loci, which occurred even in the absence of SIR3 [10512855]. | Budding yeast |
| RPL10 | Ribosomal Protein of the Large subunit 10 | Heterozygosity for RPL10 deletion increases median replicative lifespan by 24% [17174052].
| Budding yeast |
| RPL31A | Ribosomal Protein of the Large subunit 31A | Deletion of RPL31A increases mean replicative lifespan by 45% [16293764]. Mean replicative lifespan increases by 35% in the alpha strain and 50% in a strain [19030232; 18423200]. Mean replicative lifespan of the RPL31A deletion mutant increases by 35% in the ORF collection and by 29% in the remade strain [22377630]. RPL31A deletion increases significantly replicative lifespan [17174052]. Deletion of RPL31A extends replicative lifespan and is not further extended by 0.05% glucose restriction [18423200]. | Budding yeast |
| RPL6B | Ribosomal Protein of the Large subunit 6B | Deletion of RPL6B significantly increases replicative lifespan [17174052]. Replicative lifespan increases by 15% in the alpha strain and 30-40% in a strain [19030232; 18340043; 18423200]. RPL6B deletion increases replicative lifespan by 30% [16293764]. | Budding yeast |
| RPO41 | RNA POlymerase 41 | RPO41 mutants exhibit reduced chronological lifespan accompanied by imbalanced mitochondrial translation, conditional inactivation of respiration, elevated production of reactive oxygen species, and increased oxidative stress [16782871]. | Budding yeast |
| RPS18A | Ribosomal Protein of the Small subunit 18A | Deletion of both RPS18A and RPS18B paralogs increases replicative lifespan by moderately, but significantly by 15% [17174052]. Deletion of RPS18A alone does not effect mean replicative lifespan significantly [22377630]. | Budding yeast |
| RPS18B | Ribosomal Protein of the Small subunit 18B | Deletion of both RPS18A and RPS18B paralogs increases replicative lifespan moderately, but significantly by 15% [17174052]. | Budding yeast |
| RPS6B | — | RPS6B deletion increases mean replicative lifespan by about 30% [16293764]. Deletion of RPS6B, but not of the RPS6A paralog increases replicative median lifespan robustly by 45% [17174052]. | Budding yeast |
| RTG3 | ReTroGrade regulation 3 | RTG3 deletion mutation causes an increase in mean replicative in lifespan by 55% increase at 2% glucose, suggesting that expression of genes regulated by Rtg1-Rtg3 has a negative effect on longevity in 2% glucose (in YPK9). A null mutant has 123% increases mean lifespan at 0.1% glucose relative to a wild-type strain at 2% glucose, indicating that reduced glucose and an RTG3 mutation have an additive effect on lifespan (in YPK9) [11024000].
RTG3 null mutant cannot grow on acetate medium and requires glutamate or aspartate for growth [9032238]. | Budding yeast |
