Regulation of longevity and stress resistance by Sch9 in yeast.

Authors: Fabrizio P; Pozza F; Pletcher SD; Gendron CM; Longo VD

Abstract: The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates longevity and stress resistance in Caenorhabditis elegans. We screened for long-lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in life-span regulation in S. cerevisiae and higher eukaryotes.

Keywords: Adenylate Cyclase/genetics/metabolism; Amino Acid Sequence; Animals; Caenorhabditis elegans/genetics/physiology; Culture Media; Cyclic AMP-Dependent Protein Kinases/metabolism; DNA Transposable Elements; DNA-Binding Proteins/genetics/metabolism; Drosophila/genetics/physiology; Drug Resistance, Microbial; Gene Deletion; Hot Temperature; Longevity; Molecular Sequence Data; Mutagenesis, Insertional; Oxidants/pharmacology; Paraquat/pharmacology; Phenotype; Protein Kinases/chemistry/genetics/*metabolism; Saccharomyces cerevisiae/enzymology/genetics/*physiology; *Saccharomyces cerevisiae Proteins; Signal Transduction; Transcription Factors/genetics/metabolism; Transformation, Genetic
Journal: Science (New York, N.Y.)
Volume: 292
Issue: 5515
Pages: 288-90
Date: April 9, 2001
PMID: 11292860
Select reference article to upload


Citation:

Fabrizio P, Pozza F, Pletcher SD, Gendron CM, Longo VD (2001) Regulation of longevity and stress resistance by Sch9 in yeast. Science (New York, N.Y.) 292: 288-90.


Study
Update (Admin) | Auto-Update

Comment on This Data Unit