| dpy-27 | DumPY : shorter than wild-type 27 | dpy-27 RNAi in the adulthood shortens mean and maximum lifespan by 24 and 25%, respectively [23144747]. | Nematode |
| snap-1 | SNAP (Soluble NSF Attachment Protein) 1 | snap-1 RNAi in the adulthood reduces mean and maximum lifespan by 34 and 50%, respectively [23144747]. | Nematode |
| pcf-11 | cleavage and polyadenylation factor homolog 11 | pcf-11 RNAi in the adulthood decreases mean and maximum lifespan by 19 and 25% [23144747]. | Nematode |
| lys-1 | LYSozyme | lys-1 RNAi in the adulthood extends the lifespan [New longevity regulators]. Overexpression of lys-1 increases mean and maximum lifespan by 5 and 26%, but has no significant effect on median lifespan [22737090]. | Nematode |
| sptf-3 | Specificity Protein Transcription Factor 3 | RNAi against sptf-3 decreases mean and maximum lifespan by 20 - 28% and 28%, respectively. sptf-3 RNAi in the adulthood decreases the mean and maximum lifespan by 23 and 37% [23144747]. sptf-3 overexpression extends lifespan [18059442]. | Nematode |
| rab-5 | RAB family 5 | rab-5 RNAi in the adulthood reduces mean and maximum lifespan by 38 and 50% [23144747]. | Nematode |
| rpn-11 | proteasome Regulatory Particle, Non-ATPase-like 11 | rpn-11 RNAi in the adulthood decreases mean and maximum lifespan by 19 and 25%, respectively [23144747]. | Nematode |
| cdtl-7 | CDc-Two Like 7 | RNAi against cdtl-7 starting in the adulthood decreases mean and maximum lifespan by 22 and 25%, respectively [23144747]. | Nematode |
| snap-29 | SNAP (Soluble NSF Attachment Protein) homolog 29 | RNAi against snap-29 starting in the adulthood decreases mean and maximum lifespan by 49 and 72%, respectively [23144747]. | Nematode |
| ubc-18 | UBiquitin Conjugating enzyme 18 | ubc-18 overexpression is unable to extend lifespan (possibly, UBC-18 is not limiting for WWP-1 function in lifespan). Loss of ubc-18 function by mutation or RNAi reduces lifespan at 25 degree Celsius, but only slightly at 20 degree Celsius. RNAi depletion of ubc-18 completely suppresses increased longevity of eat-2 mutants. RNAi depletion of ubc-18 has no effect on long lifespan of isp-1 or daf-2 mutants. Combined knockdown of wwp-1 and ubc-18 by RNAi does not shorten lifespan any further than RNAi of either single gene. Knockdown of ubc-18 suppresses extended lifespan of wwp-1 overexpression [19553937]. | Nematode |
| aqp-1 | AQuaPorin or aquaglyceroporin related 1 | aqp-1 expression changes in response to glucose or glycerol. Similar to daf-16 and hsf-1 mutants, aqp-1 mutants were short-lived, and their short lifespan was not further decreased by glucose. Overexpression of aqp-1::GFP rescues short lifespan of aqp-1 deletion mutants and partially prevented glucose from shortening lifespan. Glucose or glycerol feeding downregulates aqp-1 in wild-type. In daf-16 and/or hsf-1 mutants aqp-1 is repressed and glucose feeding does not significantly affect its expression. aqp-1 mutation does not further decrease the short lifespan of daf-16 and/or hsf-1 mutants. aqp-1 transgene is expressed in pharynx and intestine (which behaves as entire endoderm of animal, including adipose tissues). Dietary glucose does not cause significant differences in levels of glucose or glycerol in wild-type vs. aqp-1 mutants [19883616]. | Nematode |
| nlp-7 | Neuropeptide-Like Protein | nlp-7 RNAi or overexpression reduces oxidative stress resistance and shortens lifespan of wild-type under AL. nlp-7 RNAi significantly reduces extended lifespan of eat-2 mutants, but failed to block lifespan extension of age-1 or clk-1 mutants. Lifespan of nlp-7 mutants increases only moderately by sDR [19783783]. nlp-7 expression is induced under DR via the use of a chemically defined axenic medium [17023606] and by sDR [19783783]. | Nematode |
| cup-4 | Coelomocyte UPtake defective 4 | cup-4 RNAi or overexpession reduces oxidative stress resistance and shortens lifespan of wild-type under AL. cup-4 RNAi significantly reduces the extended lifespan of eat-2 mutants, but failed to block lifespan extension of age-1 or clk-1 mutants. Lifespan of cup-4 mutants increases only moderately by sDR [19783783]. | Nematode |
| cbp-1 | CBP/p300 homolog 1 | bDR and daf-2 mutation induce cbp-1 expression. There is no decrease in cbp-1 expression in whole C. elegans during aging. Overexpression of cbp-1 does not significantly affect lifespan. daf-16 RNAi and cbp-1 RNAi reduce average lifespan under AL to about the same extent. Inhibiting cbp-1 via RNAi by 50%, specifically in adult phase and completely blocks lifespan extension of DD, bDR as well as eat-2, glp-1 and clk-1 mutation, but only partially that of daf-2 mutation and not at all that of cold. cbp-1 RNAi completely blocks the lifespan increase by daf-2 mutation under bDR. cbp-1 RNAi blocks the delay of other age-related pathologies by bDR. cbp-1 RNAi prevents protective effects of bDR and accelerates ABeta42-related pathology. bDR significantly delays onset of paralysis even in presence of cbp-1 RNAi. cbp-1 RNAi specifically in adults completely blocks lifespan extension by three distinct protocols of DR (mutation of eat-2), partly by daf-2 mutation but not of cold and blocks the delay of other age-related pathologies by bDR. cbp-1 RNAi has no effect on lifespan in daf-16 hypomorphic mutants. Combining cbp-1 and daf-16 RNAi in wild-type produces similar lifespan as either alone. Resistance to oxidative stress is strikingly reduced by cbp-1 RNAi and cbp-1 RNAi attenuates the protection against oxidative stress by bDR. cbp-1 RNAi accelerates accumulation of autofluorescence, but has no effect on activity, egg laying, or pharyngeal pumping. cbp-1 RNAi does not block induction of daf-16 or hsf-1 by bDR, but does block the induction of DAF-16 target gene, sod-3, and HSF-1 target gene, sip-1 by bDR. cbp-1 RNAi blocks induction of sod-3 expression by daf-2 RNAi. cbp-1 RNAi does not block the increased Nile Red staining produced by daf-2 mutation, but enhanced Nile Red staining. cbp-1 RNAi blocks the effect of bDR on metabolic gene expression from glycolysis towards beta-oxidation. Drugs that enhance histone acetylation increase lifespan and reduce ABeta42-related pathologies, but these protective effects are completely blocked by cbp-1 RNAi. cbp-1 RNAi decreases H4 Lys 5 acetylation and blocks the extension of lifespan as well as delays the onset of paralysis by ABeta1-42 transgene under AL and bDR by sodium butyrate (NaB) and trichostatin (TSA). cbp-1 RNAi does produce dye-filling defects in all C. elegans amphid neurons (ASI, ADL, ASK, AWB, ASH, and ASJ) [19924292]. | Nematode |
| dve-1 | DVE (Defective proVEntriculus in Drosophila) homolog) 1 | dve-1 RNAi attenuates lifespan extension by bDR, but only partially that of daf-2 mutation. dve-1 RNAi attenuates protection against oxidative stress by bDR. dve-1 expression is not induced by bDR [19924292].
| Nematode |
| phb-1 | mitochondrial ProHiBitin complex 1 | phb-1 RNAi shortens lifespan of wild-type, aak-2, cep-1 (p53) and jnk-1 mutants, but increases lifespan of daf-2, daf-7, daf-4, daf-11, gas-1, mev-1, isp-1, clk-1, nhr-49, fat-7 and gld-1 mutants. daf-16 mutation fully suppressed the exceptional long-lifespan of prohibitin-depleted (via RNAi) daf-2 mutants [19812672]. | Nematode |
| phb-2 | mitochondrial ProHiBitin complex 2 | phb-2 RNAi shortens lifespan of wild type, aak-2, cep-1 (p53) and jnk-1 mutants, but increases lifespan of daf-2, daf-7, daf-4, daf-11, gas-1, mev-1, isp-1, clk-1, nhr-49, fat-7, gld-1 mutants. daf-16 mutation fully suppressed exceptionally long-lifespan of via RNAi prohibitin-depleted, daf-2 mutants [19812672]. | Nematode |
| trx-1 | ThioRedoXin 1 | Thioredoxins regulate many cellular redox processes. trx-1 is mainly associated with neurons and is expressed in ASJ ciliated sensory neurons and to some extent also on the posterior-most internal cells. trx-1 reduces protein disulfides in the presence of a heterologous thioredoxin reductase. trx-1 null mutant display reduced mean and maximum lifespan [16387300]. Mutants with a deletion in the trx-1 gene display a decrease in lifespan and are sensitive to oxidative stress [16324156]. trx-1 overexpression extends lifespan in wild-type but not in eat-2 mutants. trx-1 deletion completely suppresses the lifespan extension caused by eat-2 mutation, but only partially suppresses that by daf-2 or osm-5 mutations. Ectopic expression of trx-1 in ASJ neurons (but not in the intestine) in trx-1 mutants rescues the lifespan-extension conferred by eat-2 mutation. trx-1 overexpression extends lifespan of wild-type but not in eat-2 mutants. trx-1 deletion almost completely suppresses lifespan extension induced by dietary deprivation (DD). DD upregulates trx-1 expression in ASJ neurons. DR activates trx-1 in ASJ neurons which in turn triggers a trx-1-dependent non-cell autonomous mechanism to extend adult lifespan [21334311].
| Nematode |
| pbs-7 | Proteasome Beta Subunit 7 | RNA interference of pbs-7 decreases lifespan of wild-type, daf-2, and glp-1 mutant [17392428]. | Nematode |
| pas-1 | — | RNAi against pas-1 started after the animal reached the late L4 stage decreases mean lifespan by 18-19% [22103665]. RNAi pas-1 decreases lifespan of daf-2 mutant, but not of WT or glp-1 mutant [17392428]. | Nematode |
| scl-20 | SCP-Like extracellular protein 20 | scl-20 appears to inhibit the growth of gld-1 tumours. RNA interference of scl-20 reduces mean lifespan up to 18% in wild-type animals, 15% in daf-2 mutants and 28% in daf-2/gld-1 mutants [17934462]. | Nematode |
| aakb-2 | AMP-Activated Kinase Beta subunit 2 | RNA interference of aakb-2 results in decreased lifespan and earlier accumulation of lipofuscin [16673436]. | Nematode |
| pnc-1 | Pyrazinamidase and NiCotinamidase 1 | pnc-1 knockdown by RNAi decreases maximum lifespan by 30%. Overexpression of pnc-1 increases adult survival under oxidative stress but does not extend the lifespan [17335870]. | Nematode |
| wwp-1 | WW domain Protein (E3 ubiquitin ligase) 1 | RNA interference of wwp-1 decreases median lifespan by 9% in wild-type animals and 24% in daf-2 mutants [18006689]. Loss of wwp-1 function by RNAi or mutation reduces lifespan at 25 degree Celsius, but not 20 degree Celsius. wwp-1 overexpression extends lifespan by up to 20%. Reduced levels of wwp-1 completely suppress the extended longevity of eat-2 mutants. Lifespan of wwp-1 mutants across entire food concentration range by bacterial dilution in liquid culture or on solid plates does not noticeable change. There is no difference in wwp-1 mRNA levels under AL and DR. RNAi reduction of pha-4, but not of daf-16 suppresses increased longevity by wwp-1 overexpression. Mutations in iron sulphur component of complex III, isp-1, increases longevity by reducing mitochondrial function. wwp-1 RNAi does not suppress the extended lifespan of isp-1 mutants and has only minor suppressive effects on lifespan of another mitochondrial mutant, clk-1, and in cyc-1 RNAi treated worms. RNAi depletion of wwp-1 has no effect on long lifespan of daf-2 mutants [19553937]. | Nematode |
| W09C5.8 | — | RNAi against W09C5.8 increases mean and maximum lifespan by 62% and 50%, respectively [12447374]. Lifespan extension by RNAi of W09C5.8 is not suppressed by daf-16. Loss of W09C5.8 activity via RNAi can also result in a shortened lifespan, reduced fertility and defects in mitochondrial respiratory chain function [19074434].
W09C5.8 RNAi animals have lower ATP content and oxygen consumption [12447374]. | Nematode |
