SRA5 encodes the low-Km cyclic AMP phosphodiesterase of Saccharomyces cerevisiae.

Authors: Wilson RB; Tatchell K

Abstract: sra5 mutations in Saccharomyces cerevisiae were previously shown to suppress the inefficient growth of ras2 strains on nonfermentable carbon sources and to result in deficient low-Km cyclic AMP (cAMP) phosphodiesterase activity. We have cloned SRA5 by complementation. It maps to the right arm of chromosome XV, tightly linked to PRT1, and its sequence matches the sequence of PDE2, encoding the low-Km cAMP phosphodiesterase. Disruptions of SRA5 allowed ras1 ras2 strains to grow either on rich media supplemented with cAMP or on minimal media without exogenous cAMP. sra5 strains failed to survive prolonged nitrogen starvation in the presence of exogenous cAMP.

Keywords: 3',5'-Cyclic-AMP Phosphodiesterases/*genetics; Chromosome Mapping; Cyclic AMP/physiology; GTP-Binding Proteins/genetics; Genes; *Genes, Fungal; Genetic Complementation Test; Mutation; Nitrogen/metabolism; Phenotype; Saccharomyces cerevisiae/enzymology/*genetics

Journal: Molecular and cellular biology Volume: 8 Issue: 1 Pages: 505-10 Date: Jan. 1, 1988 PMID: 2827010

Select reference article to upload leave this field blank to prove your humanity

Citation:

Wilson RB, Tatchell K (1988) SRA5 encodes the low-Km cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Molecular and cellular biology 8: 505-10.

Lifespan Factors:

PDE2 High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon

---

---

Back to top

---