Authors: Cui X; Wang L; Zuo P; Han Z; Fang Z; Li W; Liu J
Abstract: D-galactose causes aging acceleration in different animal models but the mechanism is unclear. In the present study, we investigated the effects of D-galactose on lifespan and oxidative stress biomarkers in the fruit fly (Drosophila melanogaster) and housefly (Musca domestica). D-galactose was added to drinking water (20 mg/ml) for housefly and to culture medium (6.5%) for fruit fly from 24 h after emergence. Oxidative stress was estimated by measuring the activity of Cu-Zn-superoxide dismutase (SOD) and the levels of lipid peroxidation products, namely malondialdehyde (MDA) and lipofuscin in housefly brain (male) and in fruit fly (male and female). D-galactose caused a significant decrease in mean lifespan (by 12.6% of male and 15.9% of female) and maximum lifespan (by 12.9% of male and 17.1% of female) in fruit fly, and also a significant decrease in mean lifespan (by 27.1% of male, 19.8% of female) and maximum lifespan (by 27.1% of male, 21.9% of female) in housefly. MDA and lipofuscin increased with age in fruit fly and in housefly brains while change of the SOD activity showed a biphasic shape with age. D-galactose caused a significant increase in MDA and lipofuscin and decrease in SOD activity in the age-matched fruit flies and houseflies. These data indicate that D-galactose shortens the lifespan of the two different fly species and that the life shortening effect is associated with an increase in oxidative stress.
Keywords: Animals; Drosophila melanogaster/*physiology; Female; Galactose/*pharmacology; Houseflies/*physiology; Life Expectancy; Lipofuscin/metabolism; Male; Malondialdehyde/metabolism; *Oxidative Stress|
Journal: Biogerontology Volume: 5 Issue: 5 Pages: 317-25 Date: Nov. 18, 2004 PMID: 15547319 |
Cui X, Wang L, Zuo P, Han Z, Fang Z, Li W, Liu J (2004) D-galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress. Biogerontology 5: 317-25.
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