Interventions

  • name effect species mean median maximum
    Jugelone treatment High jugelone concentrations led to premature death. Low juglone concentrations are tolerated well and cause a prolongation of lifespan that is associated with increased expression of small heat-shock protein HSP-16.2, enhanced glutathione levels, and nuclear translocation of DAF-16. Silencing or deletion of daf-16 prevents jugelone-induced adaptations. RNA-interference for SIR-2.1 has the same effects as daf-16 deletion but does not affect nuclear accumulation of DAF-16. DAF-16- and SIR-2.1-dependent alterations in gene expression after challenge with reactive oxygene species lead to lifespan extension [19597959]. Worm
    NAM treatment Treatment with NAM significantly decreases adult lifespan [17335870]. Worm
    L-proline supplementation L-proline supplementation increases lifespan by 5.8 and 13.6% (mean and maximum lifespan) [22482728]. Worm +6 +14
    NAC treatment Treatment with 10 mM of NAC has no effect on the lifespan of wild-type, but fully abolishes the increased longevity of nuo-6 and severly limits that of isp-1. At high concentration (> 10-15 nM) NAC can be become deleteroius even on the wild-type [21151885]. Worm
    Phloridzin treatment Administration of the apple polyphenol phloridzin at doses of 3, 10, and 30 microMolar siginificantly prolongs the replicative lifespan in K6001 strain (p < 0.01; p < 0.001). Phloridizin improves the viability of cells under oxidative stress (7 microMolar H2O2) in a dose-dependent manner and increases the significantly the expression of SOD1, SOD2, and SIR2 [21597195]. Worm
    Trehalose treatment Treatment with trehalose starting from the young-adult stage extends the mean lifespan by over 30% without any side effects. Trehalose treatment starting even from the old-adult stage shortly thereafter retards the age-associated decline in survivorship and extends the remaining lifespan by 60%. Lifespan extension by trehalose lowers the age-independent vulnerability. Trehalose increases reproductive span and retards the age-associated decrease in pharyngeal-pumping rate and the accumulation of lipofuscin autofluorescence as well as enhances thermotolerance and reduces polyglutamine. The lifespan extending effect of trehalose is abolished in daf-2 mutants [20477758]. Worm +30 to +60
    Spermidine treatment Treatment with 0.2 mM spermidine extends mean and maximum lifespan of wild-type by 16 and 13% significantly (<0.005) as well as the mean and maximum lifespan in sir-2.1(ok434) by 12 and 11% significantly (<0.01). Worm +16 +13
    Tyrosol treatment Treatment with tyrosol (250 microMolar) extends mean, median, and maximum, lifespan by 21, 21, and 11% [22824366]. Worm +21 +21 +11
    Mianserin Treatment Mianserin a serotonin receptor antagonist (used as antidepressant in humans), can increase C. elegans lifespan when given only during adulthood. Lifespan extension is reduced or abolished by mutations that affect serontonin synthesis or serotonin reuptakte at synapses [14,16]. It requires a serontonin receptor and an octopamine receptor which are both inhibited by Mianserin. Mianserin plus DR increase lifespan only by 4% more than DR alone and totally failed to extend lifepan in eat-2(ad1116) mutants. However, mianserin does not appear to reduce food intake [14]. On average, mianserin increases lifespan by 31% by an optimal dose of 50 micromolar, but had little or no effect when given at 250 micromolar. Mianserin failes to increase the lifepsna of mutants lacking serotonin synthesis enzyme TPH-1 and causes a lifespan increase of only 13% in mutant lacking serontin reuptake transporter MOD-5. Mianserin does not increase lifepan of SER-4 or SER-4 mutants. Mianserin increases lifespan by31% when given throughout adulthood, but it only result in 10% lifespan extension when it was gieven beginning at adult day 5. Mianserin also failed to increase lifespan in liquid lifespan assay and in animals grown on solid agarose plates lacking ill-defined component of commoly used agar plates (agar and Bacto peptone). Mianserin increases lifespan of animlas grown at 20 but not at 25 degree Celsius [19686215]. Worm
    Resveratrol supplementation Resveratrol supplementation prolongs the lifespan [15254550; 17460219], but not in any case [17875315]. Worm
    DDS treatment Treatment with DDS either for the entire lifetime or only during the adult period after the L4 stage extends significantly increases mean and maximum lifespan [20974969] DDS causes the delay of aging, reduces lipofuscin accumulation and decreases the level of a mitochondrial complex as well as lowers oxygen consumption and enhances oxidative stress resistance [20974969]. DDS-conferred lifespan extension is independent of daf-16 and DR (eat-2 mutants) [20974969]. Worm
    Vitamin C treatment Treatment with 1 mM vitamin C has no effect on lifespan of wild-type, but significantly shortens the lifespan of both isp-1 and muo-6 mutants [21151885]. Supplementation with vitamin C normalizes the median lifespan of wnr-1 and mir-124 mutants, which both exhibit premature aging [23075628]. Worm
    Metformin treatment Metformin treatment extends healthspan, slows lipofuscin accumulation, extends mean lifespan and prolongs healthful locomotory ability in a dose-dependent manner as well as reduces fecundity. AMPK and its activating kinase LKB1 are essential for these health benefits. Oxidative stress-responsive transcription factor SKN-1/Nrf2 is essential for metformin-confered healthspan too as it must be expressed in both neurons and intestines [20090912]. Worm
    Curcumin treatment Curcumin increases lifespan in and is associated with reduced ROS and lipofuscin during aging. Curcumin lifespan extension is attributed to its antioxidative properties. Lifespan extension had effects on body size and pharyngeal pumping rate but not on reproduction. Lifespan-extension by curcumin is abolished in osr-1, sek-1, mek-1, skn-1, unc-43, sir-2.1 and age-1 mutants, whereas curcumin treatment prolongs lifespan of mev-1 and daf-16 mutants [21855561]. *C. elegans* feed low concentration of curcumin have a decreased lipofuscin levels and enhanced the resistance to heat stress and increased mean lifespan by 39% and a maximum lifespan extended by 21.4% [23325575]. In fruit fly that survive an average of 64 days, an increase of mean lifespan to 80 days occurs in flies, with females of one strain and males of another strain experiencing an extension in lifespan. The lifespan response to curcimun exhibits variation in male and female, although the compound extends lifespan in both genders [23325575]. Worm +39 +21.4
    Propargylglycine treatment Propargylglycine (PPG) inhibits gamma-cystathioinase, the second enzyme of the trans-sulfuration pathway (TSP). PPG is a specific suicidal inhibitor of gamma-cystathionase. Gluthatione (GSH) levels are decreased by PPG administration in flies subjected to DR, whereas there is no effect on fully fed animals. PPG robustly suppresses DR lifespan extension, while longevity of fully fed flies is not affected in different strains. Thus, indicating that the effect of PPG is specific to DR. PPG abrogates changes in lifespan that are normally observed when flies are maintained in different dietary concentrations and compositions [21930912]. Fly
    PDTC treatment Treatment of Drosophila imago with PDTC increases median (by 11-13%) and maximum (by 11-14%) lifespan in females and males, respectively [22661237]. Fly +11 to +13 +11 to +14
    Simvastin treatment Treatment with simvastin significantly increases the mean and maximum lifespan and enhances cardiac function in aging animals by significantly reducing heart arrhythmias and increasing the contraction proportion o the contraction/relaxation cycle [22737247]. Fly
    L744832 treatment Farnesyl inhibitor L744832 increases lifespan [22737247]. Fly
    GGTI-298 treatment Treatment with type 1 geranylgeranyl transferase inhibitor GGTI-298 increases lifespan [22737247]. Fly
    Oligomycin treatment Oligomycin (a specific inhibitor of complex V) feeding exends lifespan on ad libitum and prevents an increase in longevity under DR (started in the adulthood) in males [19968629]. Fly
    Blueberry extract supplementation Supplementation of the diet with 5 mg/mL blueberry extract significantly extends the mean lifespan by 10% and is accompanied by an up-regulation of superoxide dismutase (SOD), catalase (CAT), and Rpn11 and down-regulationg of Methuselah (MTH). Lifespan is only extended in Oregon-R wild-type but not in SOD(n108) or Cat(n1) mutant strains [22197903]. Fly +10
    Apple polyphenol supplementation Supplemention of the diet with apple polyphenol significantly extends mean lifespan by 10% and is accompanied by up-regulation of SOD1, SOD2 and CAT as well as downregulation of MTH in aged animals [21319854]. Fly +10
    Black tea extract supplementation Supplementation of the diet with black tea extract extends the lifespan by 10% (from 51 to 56 days) and is associated with higher SOD1 and CAT expression [19770032]. Fly +10
    Cynomorium songaricum supplementation The yang-tonifying herbal medicine cynomorium songaricum Repr. (CS) supplementation to the diet extends both the mean and the maximum lifespan of adult females, but insignificantly that of males. In females, maximum lifespan (determined by the 90th survival percentile) is increased by up to 11.4% with 10 mg/mL CS and 5.7% with both 20 and 30 mg/mL Cs. Mean lifespan is significantly extended by 15, 18 and 11% upon treatment with 10, 20, and 30 mg/mL CS, respectively (all P <0.001). Increased lifespan by CS is correlated with higher resistance to oxidative stress and starvation and lower lipid hydroxyperoxids levels as well as accompanied by beneficial effects, such as improved mating readiness, increased fecundity, and suppresion of age-related learning impairment in aged animals [22844336]. Fly +11 to +18 +5.7 to +11.4
    D-chiro-inositol supplementation D-chiro-inositol supplementation to the diet extends adult longevity in both male and female animals. 20 microMolar dose of D-chiro-inositol extends median lifespan by 16.7 (p < 0.001) for males and 13% (p < 0.001) for females. Lifespan extension by D-chrio-inositol is accompanied by protection against oxidative and starvation stresses, improvement in health span, and not reduction in fecundity. Nuclear localization of foxo increases in D-chiro-inositol-fed animals [22843669]. Fly +13 to +16.7
    Interventions are an extension of GenAge and GenDR.