| Akh overexpression | Overexpression of Akh in a ubiquitousness manner enhances fat metabolism (significant increase in triglyceride synthesis and breakdown under AL), spontaneous activity (148% on AL and 154% on DR), and lifespan on AL (33%). However, despite and increase in movement under DR, lifespan is not increased under a restricted diet [22768842]. | Fly | — | +33 | — |
| Atg2 overexpression | Atg2 overexpression increases average female lifespan by 28% [18059160]. | Fly | +28 | — | — |
| Atg8a overexpression | Enhanced expression of Atg8a in older fly brains extends average adult lifespan by 56% and promotes resistance to oxidative stress [18059160]. | Fly | +56 | — | — |
| Cat overexpression | Simultaneous overexpression of catalase and Sod1 results in a one-third (i.e. 30%) lifespan extension, a slower rate of mortality acceleration, and a delayed loss in physical performance, but neither has any effect on lifespan alone [8108730]. Targeted overexpression of CAT in the mitochondrial matrix has no effect on lifespan at 25 degree Celsius, slightly shortens lifespan at 29 degree Celsius as is associated with increased resistance to endogenous hydrogen peroxide, paraquat, and cold stress [12521602]. | Fly | — | — | — |
| Cbs overexpression | Ubiquitous or neuron-specific transgenic overexpression of Cbs enhances longevity in fully-fed animals.
Adult-specific ubiquitous expression of Cbs is sufficient to increase female mean and maximum lifespan by 12 - 43% and 10%, respectively. Males, whose lifespan is relatively less affected by DR, exhibite a smaller, but still significant increase in lifespan by 7% upon Cbs overexpression. Neuronal overexpression also increases lifespan, albeit modestly (approximately 12% mean and 15% maximum lifespan extension), whereas overexpression in the fat body and in the gut has no effect [21930912]. | Fly | +12 to +43 | — | +10 to +15 |
| Cct1 overexpression | Overexpression of Cct1 from a doxycycline-inducible promoter results in a 6 - 8% increase in mean lifespan (in the PdL x rtTA; Oregon-R x rtTA strain) [12620118]. | Fly | +6 to +8 | — | — |
| CG10383 overexpression | Overexpression of CG10383 in males increases mean and maximum lifespan by 12% and 8%, respectively [22366109]. | Fly | +12 | — | +8 |
| CG10916 overexpression | Overexpression of CG10916 in males increases mean and maximum lifespan by 27% and 26%, respectively [22366109]. | Fly | +27 | — | +26 |
| CG13890 overexpression | Overexpression of CG13890 (DCI) throughout the whole body increases mean and median lifespan by 35 and 31%, but decreases maximum lifespan by 6%, increases stress resistant (to paraquat and starvation), consistently reduces the mortality rate across adult ages and reduces the lifespan extension of DR by 15% [22997544].
CG6783 overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of CG6783 [22997544]. | Fly | +35 | +31 | +6 |
| CG30427 overexpression | Overexpression of CG30427 in males increases mean lifespan by 18% [22366109]. | Fly | +18 | — | — |
| CG3776 overexpression | Overexpression of CG3776 (alias Jhebp29) reduces the mean lifespan, where the reduction in males is slightly higher.
The lifespan of female and male flies with overexpressed CG3776 is reduced by 35% and 42.6%, respectively when compared with Oregon R flies [18275960].
| Fly | -35 to -42.6 | — | — |
| cher overexpression | Overexpression of cher from a doxycycline-inducible promoter results in a 7 - 9% increase in mean lifespan (in PdL x rtTA; Oregon-R x rtTA) [12620118]. | Fly | +7 to +9 | — | — |
| Constitutive active S6k overexpression | Overexpression of a constitutively active form of S6k (alias dS6K) decreases mean lifespan by 34% at 29°C [15186745]. | Fly | -34 | — | — |
| Constitutive miR-277 expression | Constitutive miR-277 expression shortens lifespan and synthetically lethal with reduced insulin signaling, indicating that metabolic control underlies this phenotype [23669073]. | Fly | — | — | — |
| DNApol-gamma35 overexpression | Overexpression of DNApol-gamma35 (DNA polymerase gamma) in the nervous system results in a decrease in the median lifespan ranging from 39% to 52% [17999718]. | Fly | -39 to -52 | — | — |
| Dominant-negative S6k | Ubiquitous overexpression of a dominant-negative form of S6k (alias dS6K) increases mean lifespan by 22%. Overexpression of a dominant-negative form of S6k protects mutants from deleterious effects of rich food, as if mimicking the effect of DR [15186745]. | Fly | +22 | — | — |
| Ef1alpha48D overexpression | Overexpression of Ef1alpha48D (transformed with a P-element vector and under control of hsp70 regulatory sequences) results in lifespan extension by 18-41% [2508089]. | Fly | +18 to +41 | — | — |
| Eip71CD overexpression | Overexpression of Eip71CD (alias MsrA) in nervous system extends the lifespan by up to 70%, increases resistance to oxidative stress, and delays the onset of senescence-induced decline in activity levels and reproductive capacity. Eip71CD is a downstream effector of foxo [22310715].
Mean and maximum lifespan is increased by up to 2-% in animals that overexpress Eip71CD [20655917]. | Fly | +20 to 70 | — | +20 |
| fabp overexpression | Overexpression of fabp (CG6783) throughout the whole body increases mean, median and maximum lifespan by 77, 81 and 13%, increases stress resistant (to paraquat but not starvation), consistently reduces mortality rate across adult ages and reduces the lifespan extension of DR by 12% [22997544].
fabp overexpression increases the dFOXO nuclear localization in the fat-body. mRNA levels of dFOXO target genes l(2)efl and 4E-BP in the adult whole bodies increases in response to overexpression of fabp [22997544].
Females of the genotype Act-GS-Gal4 > UAS-CG6783 exhibit an increase in median lifespan compared to uninduced control in response to feeding with RU486-containing food from day 3 of adulthood (P < 0.0001). Mean lifespan is extended by 10, while maximum lifespan is decreased by 11% [22997544]. | Fly | +77 | +81 | +13 |
| fh overexpression | Overexpression of fh in the mitochondria of female transgenic animals increases antioxidant capability, resistance to oxidative stress insults, and longevity [18258192]. | Fly | — | — | — |
| foxo overexpression | foxo overexpression extends lifespan. Activation of foxo in the adult pericerbral fat body is sufficient for lifespan extension [15175753]. Overexpression of foxo in the adult adipose tissue alone prolongs lifespan [15192154; 15175753]. Limited activation of foxo reduces the expression of Drosophila insulin-like peptide dilp-2 synthesized in neurons and, represses endogenous insulin-dependent signaling in peripheral fat body [15175753]. foxo overexpression in adult fat body under normal nutritional conditions leads to extension of lifespan of females and causes a right shift of the response curve of lifespan to DR [18241326].
Overexpression of dFOXO in adult fat body increases median, by 21-33%, and maximum lifespan as well as lowers the age-specific mortality at all ages, in two independent experiments. Overexpression of dFOXO increases lifespan by lowering the whole mortality trajectory, with no effect on slope (similar to DR). Initiation of dFOXO expression at different ages increases subsequent lifespan with the magnitude of increase decreasing as the animals were put on RU486 (which activates the foxo transgene via UAS) at older ages. The effects of removal of dFOXO overexpression at different ages closely mirrored those of induction of expression and produce shortest lifespan observed in animals taken of RU486 at the earlier ages [17465980]. | Fly | — | +21 to +33 | — |
| Gadd45 overexpression | Gadd45 overexpression in the nervous system leads to a significant increase of lifespan without a decrease in fecundity and locomotor activity. The lifespan extension effect is more pronounced in males than in females. Additional maximum lifespan is also extended. The maximum lifespan is increased by 50% and 59% for females and males, respectively. The median lifespan is extended by 46 and 77% for females and males, respectively [22661237]. | Fly | — | — | — |
| Gclc overexpression | Overexpression of Gclc extended mean and maximum lifespan by up to 50% [16148000]. | Fly | +50 | — | +50 |
| Gclm overexpression | Overexpression of Gclm extends the mean lifespan by up to 24% [16148000]. | Fly | +24 | — | — |
| gig overexpression | Overexpression of gig, also known as dTsc2, results in lifespan extension. Overexpression of dTsc2 increases mean lifespan by 20% and 12%, at 25°C and 29°C, respectively, and protects from deleterious effects of rich food, as if mimicking the effect of DR [15186745].
Overexpression of dTsc2 via a UAS promoter in the eye using the driver gmr-GAL4 or in the nervous system by using appI-GAL4 does not extend the lifespan. Using the drivers 24BGAL4 and PO188-GAL4, enhancer traps that are predominantly expressed in the muscle and fat results in mean lifespan extension of 27% and 37%, respectively, at 29°C [15186745]. Fat-specific drivers DJ634-GAL4 and PO163-GAL4 when used to overexpress dTsc2, also led to a mean lifespan extension of 22% and 31%, respectively, at 29°C [15186745]. | Fly | +20 to +31 | — | — |
GenAge
GenDR
