Oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) for just about 7 months to rats not only does not entail chronic toxicity but it almost doubles the lifespan. The effects on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours [22498298].

C60-olive oil can increase the mean, median and maximum lifespan by 114, 91 and 74%. C60-olive oil extends the lifespan of animals with a probability of 0.999 and 0.995 with respect to water and olive oil treatments, respectively [22498298].

The GSSG/GSH ratio of animals treated by C60-olive oil is significantly less (about twice as less) as compared to controls [22498298].

C60 solutions have a characteristic purple color. C60 can cross the blood-brain barrier [21787853]. The elimination process follows a non-urinary route as it is mainly eliminated through the bile ducts [16351219; 21787853]. C60 reacts inside the liver cells with vitamin A following a Diels-Alder like reaction both in mice and rats [16351219].