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Symbol: cnb-1 Name: CalciNeurin B 1 Entrez gene ID: 179572 Ensembl gene ID: F55C10.1 Species: Worm (Taxid: 6239) Functional description: Protein CNB-1; CalciNeurin B family member (cnb-1) [Source:RefSeq peptide;Acc:NP_505885] [Ensembl]; cnb-1 encodes an ortholog of calcineurin B, the regulatory subunit of the protein phosphatase 2B with four EF-hand motifs for calcium binding, that binds TAX-6 (a calcineurin A ortholog) in a calcium-dependent manner; CNB-1 binds calcium, enhances the phosphatase activity of TAX-6 in vitro, promotes transcription of rcn-1, and is required for normal cuticle formation, sperm morphology, and brood size; cnb-1 null mutants exhibit an extended lifespan which is characterized by enhanced autophagy and dependent upon the presence of functional bec-1 and atg-7. At the surface of the worm, cnb-1 mutants show thinner cuticle than usual, resulting in animals having a somewhat clear (Clr; transparent) phenotype. cnb-1 mutants also have decreased brood sizes, smaller and thinner bodies than normal, delayed egg-laying, and uncoordinated or lethargic movement. cnb-1 mutant phenotypes resemble those of the gain-of-function calmodulin mutation unc-43(n498). Double mutants of unc-43(lf) and cnb-1 arrest in early larval stages with severe morphological defects. However, double unc-43(gf);cnb-1 mutants are viable, and have movement, fertility, egg-laying, and drug-resistance phenotypes stronger than those seen with individual unc-43(gf) or cnb-1 mutations. Mutation of cnb-1 lowers expression of an rcn-1::GFP transgene, which is thought to reflect transcriptional activation of rcn-1 by cnb-1 activity in vivo. cnb-1 mutants have abnormal sperm morphology (smaller and smoother with smaller psuedopods than wild-type), and oocyte debris consistent with a possible endomitotic oocyte (Emo) defect arising from spermatheca defects. Mating with wild-type males does not fully rescue low brood size, indicating that oocyte defects significantly contribute to reduced fertility. [WormBase] Observation: RNA interference of cnb-1 increases mean lifespan by 30% [17673661]. Interventions: Assays: Organismal Lifespan Classification: Aging Relevance Analysis/Source:  GenAge GenDRHomologs Edit \ Update (Admin) | Delete |
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