Authors: Bertram MJ; Bérubé NG; Hang-Swanson X; Ran Q; Leung JK; Bryce S; Spurgers K; Bick RJ; Baldini A; Ning Y; Clark LJ; Parkinson EK; Barrett JC; Smith JR; Pereira-Smith OM
Abstract: Based on the dominance of cellular senescence over immortality, immortal human cell lines have been assigned to four complementation groups for indefinite division. Human chromosomes carrying senescence genes have been identified, including chromosome 4. We report the cloning and identification of a gene, mortality factor 4 (MORF 4), which induces a senescent-like phenotype in immortal cell lines assigned to complementation group B with concomitant changes in two markers for senescence. MORF 4 is a member of a novel family of genes with transcription factor-like motifs. We present here the sequences of the seven family members, their chromosomal locations, and a partial characterization of the three members that are expressed. Elucidation of the mechanism of action of these genes should enhance our understanding of growth regulation and cellular aging.
Keywords: Amino Acid Sequence; Base Sequence; Cell Aging/*genetics; Cell Division/genetics; Cell Line; Cell Nucleus/metabolism; Gene Expression; Genetic Complementation Test; Humans; Molecular Sequence Data; Multigene Family; Oligonucleotide Probes/genetics; Phenotype; RNA, Messenger/genetics/metabolism; Sequence Homology, Amino Acid; Transcription Factors/*genetics/metabolism|
Journal: Molecular and cellular biology Volume: 19 Issue: 2 Pages: 1479-85 Date: Jan. 16, 1999 PMID: 9891081 |
Bertram MJ, Bérubé NG, Hang-Swanson X, Ran Q, Leung JK, Bryce S, Spurgers K, Bick RJ, Baldini A, Ning Y, Clark LJ, Parkinson EK, Barrett JC, Smith JR, Pereira-Smith OM (1999) Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes. Molecular and cellular biology 19: 1479-85.
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