Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast.

Authors: Aerts AM; François IE; Bammens L; Cammue BP; Smets B; Winderickx J; Accardo S; De Vos DE; Thevissen K

Abstract: The antifungal plant defensin DmAMP1 interacts with fungal sphingolipids of mannosyldiinositolphosphorylceramide (M(IP)2C) class. We screened a Saccharomyces cerevisiae transposon (Tn) mutant library against DmAMP1 and identified one DmAMP1-resistant mutant with the Tn inserted in the M(IP)2C biosynthesis gene IPT1 (DmTn11) and one DmAMP1-hypersensitive mutant with the Tn inserted in rDNA (HsTnII). However, tetrad analysis pointed to HsTnII as a spontaneous mutant. Apparently, membranes of DmTn11 lack M(IP)2C, whereas membranes of HsTnII have increased M(IP)2C levels. In addition, DmTn11 and HsTnII are characterized by increased and reduced oxidative stress resistance/chronological life-span (CL), respectively. A putative involvement of M(IP)2C in oxidative stress and CL in yeast is discussed.

Keywords: Defensins/*physiology; Glycosphingolipids/analysis/*physiology; Mutation; *Oxidative Stress; Oxygen/*metabolism; Plants/immunology; Saccharomyces cerevisiae/cytology/genetics; Yeasts/*cytology
Journal: FEBS letters
Volume: 580
Issue: 7
Pages: 1903-7
Date: March 11, 2006
PMID: 16527275
Select reference article to upload


Citation:

Aerts AM, François IE, Bammens L, Cammue BP, Smets B, Winderickx J, Accardo S, De Vos DE, Thevissen K (2006) Level of M(IP)2C sphingolipid affects plant defensin sensitivity, oxidative stress resistance and chronological life-span in yeast. FEBS letters 580: 1903-7.


Study
Update (Admin) | Auto-Update

Comment on This Data Unit