Authors: Calin GA; Dumitru CD; Shimizu M; Bichi R; Zupo S; Noch E; Aldler H; Rattan S; Keating M; Rai K; Rassenti L; Kipps T; Negrini M; Bullrich F; Croce CM
Abstract: Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.
Keywords: Adult; Antigens, CD5/analysis; B-Lymphocytes/chemistry/cytology; Blotting, Northern; Blotting, Western; Child; Child, Preschool; Chromosomes, Human, Pair 13/*genetics; *Gene Deletion; *Gene Expression Regulation, Leukemic; Humans; Hybrid Cells/chemistry; Leukemia, Lymphocytic, Chronic, B-Cell/*genetics; MicroRNAs/biosynthesis/*genetics; Middle Aged; Neoplasms/genetics/pathology; Neoplastic Stem Cells/chemistry; Palatine Tonsil/cytology; RNA, Neoplasm/biosynthesis/*genetics; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic; Tumor Cells, Cultured/chemistry|
Journal: Proceedings of the National Academy of Sciences of the United States of America Volume: 99 Issue: 24 Pages: 15524-9 Date: Nov. 16, 2002 PMID: 12434020 |
Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K, Rassenti L, Kipps T, Negrini M, Bullrich F, Croce CM (2002) Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proceedings of the National Academy of Sciences of the United States of America 99: 15524-9.
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