A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila.

Authors: Tsuda M; Sugiura T; Ishii T; Ishii N; Aigaki T

Abstract: Oxidative stress is thought to promote aging by damaging various cellular components. We investigated the phenotypic effects of mutation in the sdhC gene encoding succinate dehydrogenase C in Drosophila by using transgenic flies expressing a dominant-negative form, SdhC(I71E). Expression of SdhC(I71E) significantly reduced the mean lifespan by 22% compared to that of control flies. The amount of protein carbonyl was significantly increased, suggesting that a high level of oxidative stress was induced in these flies. The transgenic flies should serve as a model for studying the biological processes associated with increased levels of oxidative stress and mitochondrial dysfunction in Drosophila.

Keywords: Animals; Bacterial Proteins/genetics/*metabolism; Caenorhabditis elegans Proteins/*metabolism; Drosophila/*physiology; Longevity/*physiology; Oxidative Stress/*physiology; Succinate Dehydrogenase/*metabolism
Journal: Biochemical and biophysical research communications
Volume: 363
Issue: 2
Pages: 342-6
Date: Sept. 15, 2007
PMID: 17854771
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Citation:

Tsuda M, Sugiura T, Ishii T, Ishii N, Aigaki T (2007) A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila. Biochemical and biophysical research communications 363: 342-6.


Lifespan Factors:
  • sdhC succinate dehydrogenase, cytochrome b556 subunit


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