Rapid accumulation of fluorescent material with aging in an oxygen-sensitive mutant mev-1 of Caenorhabditis elegans.

Authors: Hosokawa, H; Ishii, N; Ishida, H; Ichimori, K; Nakazawa, H; Suzuki, K

Abstract: Mutations in mev-1 of the nematode Caenorhabditis elegans render animals hypersensitive to high oxygen concentrations. They also reduce life span. To further understand the effects of mev-1 on aging, accumulation of fluorescent material resembling lipofuscin was measured by biochemical and histological analyses. Fluorescent material accumulated in both wild type and mev-1 animals with increasing age. The mev-1 mutant accumulated more fluorescent material at a greater rate than dose wild type. Furthermore, the accumulation rates depended on concentration of oxygen. Since this phenotype has been widely used as an aging marker, these results validate mev-1's use as a model to study aging.

Keywords: Aging/*metabolism; Animals; Caenorhabditis elegans/drug effects/*genetics/*metabolism; Drug Resistance; Fluorescence; Fluorometry; *Mutation; Oxygen/*pharmacology; Reference Values
Journal: Mech Ageing Dev
Volume: 74
Issue: 3
Pages: 161-70
Date: June 1, 1994
PMID: 7934213
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Citation:

Hosokawa, H, Ishii, N, Ishida, H, Ichimori, K, Nakazawa, H, Suzuki, K (1994) Rapid accumulation of fluorescent material with aging in an oxygen-sensitive mutant mev-1 of Caenorhabditis elegans. Mech Ageing Dev 74: 161-70.


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