A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis

Plant Cell. 2002 Apr;14(4):805-15. doi: 10.1105/tpc.010422.

Abstract

SAG101, a leaf senescence-associated gene, was cloned from an Arabidopsis leaf senescence enhancer trap line and functionally characterized. Reporter gene and RNA gel blot analyses revealed that SAG101 was not expressed until the onset of senescence in leaves. A recombinant SAG101 fusion protein overexpressed in Escherichia coli displayed acyl hydrolase activity. Antisense RNA interference in transgenic plants delayed the onset of leaf senescence for approximately 4 days. Chemically induced overexpression of SAG101 caused precocious senescence in both attached and detached leaves of transgenic Arabidopsis plants. These data suggest that SAG101 plays a significant role in leaf senescence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis Proteins / genetics*
  • Carboxylic Ester Hydrolases / genetics*
  • Chlorophyll / metabolism
  • Cloning, Molecular
  • DNA, Antisense / genetics
  • DNA, Antisense / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / metabolism
  • Dexamethasone / pharmacology
  • Escherichia coli / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Molecular Sequence Data
  • Phenotype
  • Plant Leaves / enzymology
  • Plant Leaves / genetics*
  • Plant Leaves / growth & development
  • Plants, Genetically Modified
  • RNA-Binding Proteins / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Rhizobium / genetics
  • Thiolester Hydrolases / genetics*
  • Thiolester Hydrolases / metabolism
  • Ubiquitin-Protein Ligases

Substances

  • Arabidopsis Proteins
  • DNA, Antisense
  • DNA, Complementary
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Chlorophyll
  • Dexamethasone
  • RNF7 protein, human
  • Ubiquitin-Protein Ligases
  • Carboxylic Ester Hydrolases
  • SAG101 protein, Arabidopsis
  • Thiolester Hydrolases
  • Glucuronidase

Associated data

  • GENBANK/AF239887
  • GENBANK/AF239888